Acinetobacter baumannii real-time PCR Assay

A PathoSeek research-use real-time PCR assay for A. baumannii detection in environmental monitoring and infection-control research.

Product code: DDN-AB-004 Research Use Only
Acinetobacter baumannii real-time PCR Assay kit package
Available in 50 or 100 reaction kits

Ordering, documentation, and research-use details.

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Ordering information

Product code: DDN-AB-004. Lead time may apply.

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Ordering details

50 reactions per kit. Contact Delphine Diagnostics for pricing, availability, and lead time.

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Recommended equipment

Runs on standard real-time PCR platforms including ABI 7500, Bio-Rad CFX96, Roche LightCycler, and Rotor-Gene Q.

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Documentation

Product insert, Safety Data Sheet, and approved Instructions for Use can be provided with product documentation.

Shared PathoSeek assay characteristics.

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Multiplex, qualitative real-time PCR using TaqMan hydrolysis-probe chemistry.

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All targets are detected simultaneously in a single reaction and reported as detected or not detected.

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50 reactions per kit.

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High sensitivity: detects fewer than 100 copies of target with greater than 95% priming efficiency.

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Results in a few hours compared with several days for traditional culture.

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Built-in positive, negative, and RNase P internal controls.

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Runs on standard real-time PCR platforms including ABI 7500, Bio-Rad CFX96, Roche LightCycler, and Rotor-Gene Q.

Detection sections available across all four products.

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Bacterial Pathogen Detection

Targets
Escherichia coli, Acinetobacter baumannii, Staphylococcus aureus
Description
A real-time PCR panel for rapid detection of three widely studied bacterial pathogens associated with healthcare-associated infections, environmental contamination, and antimicrobial-resistance research. Suited to infection-control studies, environmental monitoring, and microbial-persistence models.
Key note
Detects bacterial DNA only and does not identify resistance markers, including methicillin resistance.
02

Respiratory Pathogen Detection

Targets
Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae
Description
A real-time PCR panel targeting three major bacteria linked to pneumonia, bloodstream infections, and meningitis. Supports research into bacterial persistence, transmission pathways, and environmental contamination.
Key note
Does not provide antimicrobial-resistance or virulence-factor data.
03

Antimicrobial Resistance (AMR) Gene Detection

Targets
NDM, CTX-M, mecA
Description
A real-time PCR panel for detecting three key antimicrobial-resistance genes: NDM (resistance to many last-line antibiotics, including carbapenems), CTX-M (resistance to penicillins and broad-spectrum cephalosporins), and mecA (the primary marker associated with MRSA). Built for resistance surveillance, transmission-dynamics, and environmental-reservoir research.
Key note
Detecting a resistance gene does not confirm phenotypic resistance, gene expression, or bacterial viability.
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Pathogen + Resistance Marker Detection

Targets
Candida auris, OXA-48, Van A/B
Description
A combined real-time PCR panel detecting the emerging multidrug-resistant yeast Candida auris alongside two resistance markers: OXA-48 (carbapenem resistance) and vanA/vanB (vancomycin resistance). Aimed at surveillance, infection-control, and AMR research.
Key note
Detecting OXA-48 or vanA/vanB does not confirm phenotypic resistance without additional testing.
Acinetobacter baumannii
Acinetobacter baumannii

Acinetobacter baumannii

Acinetobacter baumannii is a Gram-negative bacterium, notorious for causing severe healthcare-associated infections, including ventilator-associated pneumonia, bloodstream infections, and wound infections.

A. baumannii is known for developing antibiotic resistance and surviving on surfaces, making it difficult to control, and can have mortality rates of up to 70%. This kit helps detect A. baumannii and is suitable for environmental monitoring, infection control research, and experimental models of bacterial transmission.

More Information

Client feedback requested downloadable IFU content for PathoSeek. The sections below now include available shared product details from the feedback document; final reaction volumes, thermal cycling values, component quantities, storage temperatures, and troubleshooting rows should be completed from the approved IFU PDF.

Reaction setup

Reaction setup volumes and total reaction volume

Multiplex, qualitative real-time PCR using TaqMan hydrolysis-probe chemistry. Targets are detected simultaneously in a single reaction and reported as detected or not detected.

Thermal cycling

Temperature profile and cycle counts

Runs on standard real-time PCR platforms including ABI 7500, Bio-Rad CFX96, Roche LightCycler, and Rotor-Gene Q. Final temperature and cycle-count values should come from the approved IFU.

Kit components

Vials, quantities, and storage temperatures

50 reactions per kit with built-in positive, negative, and RNase P internal controls. Final vial quantities and storage temperatures should come from the approved IFU.

Troubleshooting

Troubleshooting table

High sensitivity: detects fewer than 100 copies of target with greater than 95% priming efficiency. Final troubleshooting causes, corrective actions, and acceptance criteria should come from the approved IFU.

Request product details for Acinetobacter baumannii real-time PCR Assay.

Contact Delphine Diagnostics for pricing, availability, lead time, documentation, and IFU updates.

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Research Use Only

This PathoSeek product is presented for research use only and is not described here as a diagnostic product for clinical decision-making.